Determine the dilution factor and mixing ratio for preparing a buffer from a concentrated stock. Enter the stock and target concentrations along with the desired final volume. The calculator reports the fold dilution, stock volume, diluent volume, and stock fraction, making it easy to scale a recipe without changing concentration.
The main result states how many-fold the stock must be diluted to reach the target concentration.
The calculation assumes the stock contains all relevant components at the stated multiple and that simple volume-based dilution is appropriate.
A 10× stock diluted to 1× has a 10-fold dilution factor. For 1000 mL final volume, combine 100 mL stock with approximately 900 mL diluent.
One part stock is brought to ten parts total volume, which is approximately one part stock plus nine parts diluent.
Yes. The dilution factor is 1 and no diluent is required.
Dilution factor describes how many times more concentrated the stock is than the working solution.
Yes, provided both stock and target use the same percent basis, such as both w/v.
No. Increase the entered final volume if you need extra buffer for dead volume or transfer loss.
| Variable | Meaning | Unit |
|---|---|---|
| Cstock | Stock concentration or strength | same as target |
| Ctarget | Required working concentration | same as stock |
| DF | Cstock ÷ Ctarget | fold |
| Vfinal | Final working volume | mL |