Calculate the dilution factor and solvent volume needed to prepare a PCR working solution from a more concentrated stock. Enter stock concentration, desired working concentration, and final volume to obtain the stock aliquot, diluent volume, dilution ratio, and achievable number of preparations.
Using C₁V₁ = C₂V₂: stock volume V₁ = target concentration × final volume ÷ stock concentration. Dilution factor = stock concentration ÷ target concentration. Diluent volume = final volume − stock volume.
Use the main result as a planning estimate and read the supporting metrics to understand which assumptions drive it.
Results depend on the entered assumptions. Confirm critical decisions with measured operational data or a validated laboratory protocol.
Diluting a 10× stock to 1× with a 1,000 µL final volume requires 100 µL of stock and 900 µL of diluent, a 10-fold dilution.
It uses only the values entered on the page and the formula shown above. Review the inputs whenever operating conditions or lab protocols change.
Use measured averages where possible and include the provided overage, utilization, overlap, or efficiency input rather than hiding variability in another field.
Zero is accepted only where it has a meaningful interpretation. The calculator blocks zero denominators and other combinations that cannot produce a valid result.
No. It is a planning aid. Confirm reagent compatibility, instrument limits, assay performance, and pipetting feasibility with your validated protocol.
Keep full precision through the calculation and round only the quantities you need to measure or report. The calculator follows that approach internally.
| Input | Unit | Role |
|---|---|---|
| Stock concentration | × | Concentration in any consistent unit |
| Target concentration | × | Use the same unit as stock |
| Final working volume | µL | Volume to prepare |
| Available stock volume | µL | Optional capacity check |