Calculate the final concentration of a PCR component after adding a stock solution to a reaction. Use any consistent concentration unit and enter the stock concentration, aliquot volume, and final reaction volume to see the resulting concentration, dilution factor, and remaining volume for other components.
Final concentration = stock concentration × stock aliquot volume ÷ final reaction volume. Dilution factor = final reaction volume ÷ stock aliquot volume.
Use the main result as a planning estimate and read the supporting metrics to understand which assumptions drive it.
Results depend on the entered assumptions. Confirm critical decisions with measured operational data or a validated laboratory protocol.
Adding 1 µL of a 10 µM primer stock to a 20 µL PCR reaction gives a 0.5 µM final concentration, a 20-fold dilution.
It uses only the values entered on the page and the formula shown above. Review the inputs whenever operating conditions or lab protocols change.
Use measured averages where possible and include the provided overage, utilization, overlap, or efficiency input rather than hiding variability in another field.
Zero is accepted only where it has a meaningful interpretation. The calculator blocks zero denominators and other combinations that cannot produce a valid result.
No. It is a planning aid. Confirm reagent compatibility, instrument limits, assay performance, and pipetting feasibility with your validated protocol.
Keep full precision through the calculation and round only the quantities you need to measure or report. The calculator follows that approach internally.
| Input | Unit | Role |
|---|---|---|
| Stock concentration | µM | Change the interpretation if using another unit |
| Stock volume added | µL | Aliquot placed in each reaction |
| Final reaction volume | µL | Total volume after all components |