Plan a one-step DNA dilution using the measured stock concentration, desired working concentration, and final volume. The calculator reports the concentration dilution factor, the stock aliquot to transfer, and the diluent volume to add. It follows C₁V₁ = C₂V₂ and rejects targets above the stock concentration because a simple dilution cannot concentrate a sample.
Dilution factor = Stock concentration ÷ Target concentration
Stock volume = Target concentration × Final volume ÷ Stock concentration
Diluent = Final volume − Stock volume
The dilution factor describes how many total parts the final solution contains for each one part of stock.
If the calculated stock aliquot is below the reliable range of your pipette, use an intermediate dilution rather than attempting the transfer directly.
To make 100 µL of 20 ng/µL DNA from a 200 ng/µL stock, the factor is 200 ÷ 20 = 10×. Transfer 10 µL stock and add 90 µL diluent.
No. Use the same volume unit throughout so the volume ratio remains valid.
Adding diluent cannot raise concentration; concentration requires a different process.
Yes. Serial steps can make tiny stock transfers more practical and reproducible.
No. It calculates nominal volumes; add a justified preparation allowance separately if needed.
Prepare an intermediate dilution or increase the final volume instead of relying on an unreliable transfer.
| Output | Calculation |
|---|---|
| Dilution factor | C₁ ÷ C₂ |
| Stock aliquot | C₂ × V₂ ÷ C₁ |
| Diluent | V₂ − V₁ |
| Mass check | C₁V₁ = C₂V₂ |