Estimate how many complete samples can be processed from an available reagent mix for DNA assay reactions. The calculation first removes a user-defined reserve for transfer loss, converts the remaining volume to whole reactions, assigns control reactions, and groups the balance by the requested replicate count. Capacity is rounded down so the result never promises a partial sample.
Usable volume = Available volume × (1 − Reserve %)
Samples = floor((floor(Usable volume ÷ Volume per reaction) − Controls) ÷ Replicates)
The main result is the number of samples that can each receive the full replicate allocation after controls.
Confirm whether the entered per-reaction volume is the entire reaction or only the limited reagent mix being budgeted.
With 1,000 µL available, a 10% reserve leaves 900 µL. At 20 µL per reaction that supports 45 reactions. After 4 controls, 41 remain; at 3 replicates per sample, capacity is 13 complete samples.
A partial replicate set cannot complete another sample, so only whole fully allocated samples are reported.
No. Controls are deducted before the remaining reactions are grouped into samples.
It reduces the volume treated as usable; it is an allowance for handling loss.
Enter one replicate per sample.
Yes. The calculator will report zero capacity without producing an invalid result.
| Step | Operation |
|---|---|
| Reserve | Reduce available volume by the entered percentage |
| Reactions | Round usable volume ÷ per-reaction volume down |
| Controls | Subtract dedicated control wells |
| Samples | Divide remaining wells by replicates and round down |