Plan the total volume of a single reagent needed for a RNA concentration workflow. Enter the sample count, technical replicates, control reactions, reagent volume per reaction, and a preparation overage. The calculator separates the exact base requirement from the additional transfer-loss allowance so a batch can be prepared consistently without treating the overage as extra reactions.
Planned reactions = Samples × Replicates + Controls
Total reagent = Planned reactions × Reagent per reaction × (1 + Overage %)
The main result is the volume to prepare for the specified reagent, including the entered overage.
Calculate each reagent separately if components have different per-reaction volumes. Overage does not replace required controls.
For 24 samples, 1 replicate, 2 controls, and 5 µL per reaction, the base need is 26 × 5 = 130 µL. With 10% overage, prepare 143 µL.
Yes. Control reactions are added to sample reactions before reagent volume is calculated.
No. Overage is a percentage of total base volume and may not equal one full reaction.
Only if the per-reaction value represents the entire mix; otherwise calculate each component separately.
They represent discrete planned reactions and cannot be fractional.
Only if you represent it through the entered overage percentage.
| Input | Purpose |
|---|---|
| Samples × replicates | Sample reaction demand |
| Controls | Standards, blanks, and controls |
| Volume per reaction | Protocol amount of this reagent |
| Overage | Allowance for handling loss |