Convert a protein assay absorbance into sample concentration using a linear standard-curve slope and intercept, with blank correction and dilution correction kept explicit. The result also shows the corrected absorbance and diluted-tube concentration for quick verification against your fitted curve.
The output estimates the original sample concentration from a linear calibration model. Confirm that the corrected signal lies within the standards used to fit the curve.
Do not extrapolate far beyond the standard range. Use the same blanking convention that was used when the slope and intercept were fitted.
With absorbance 0.82, blank 0.05, intercept 0.02, slope 0.25 AU per mg/mL, and a 10× dilution, the original concentration is 30 mg/mL.
It uses the numerical inputs shown on the page and does not supply an instrument-specific calibration or laboratory standard.
Yes when every concentration input and curve coefficient are expressed in a mutually consistent unit system.
The blank estimates background signal. Subtracting it prevents background from being attributed to the sample.
No. It is a planning or curve-based estimate and should be checked against the applicable assay or measurement procedure.
Dilute or repeat the sample so its signal falls within the validated range rather than relying on distant extrapolation.
| Field | Meaning |
|---|---|
| Diluted \1Onc | Calculated output |
| Corrected \1Bs | Calculated output |
| Net \1Ignal | Calculated output |