Plan protein assay working reagent for samples, standards, blanks, and technical replicates. A configurable excess percentage covers routine pipetting loss, while the output separates base demand from overage so you can prepare enough reagent without hiding the assumptions.
The main result is the working reagent volume to prepare. It includes every planned replicate and the selected overage.
Check the assay kit instructions for reagent ratios and maximum plate volume; this calculator does not define the chemistry.
For 24 samples, 10 control wells, duplicates, 200 µL per well, and 10% overage: 68 wells require 13.6 mL base volume and 14.96 mL total.
Overage covers expected pipetting, transfer, and vessel losses. It should reflect the workflow rather than being treated as a universal recommendation.
It is the total selected reagent or working mixture described by the inputs. Component ratios must still follow the relevant protocol.
Use the units shown in the fields. Convert inputs first so every volume in the same calculation uses the stated unit.
No. Confirm well, tube, rotor, and vessel limits separately before preparing or loading material.
Separating base demand from extra volume makes the preparation assumption visible and easier to document.
| Field | Meaning |
|---|---|
| Total \1Ells | Calculated output |
| Base \1Olume | Calculated output |
| Extra \1Olume | Calculated output |