Calculate a protein assay dilution from sample and diluent volumes, then convert an assay-measured concentration back to the original sample concentration. The calculator reports total volume, dilution factor, and sample fraction to make serial-dilution records easier to check.
A 10× dilution means one part sample in ten parts total volume. Multiply the diluted assay result by 10 to estimate the original concentration.
For multiple serial steps, multiply the dilution factors for every step before correcting the measured concentration.
Mixing 20 µL sample with 180 µL diluent creates 200 µL total and a 10× dilution. A measured 0.35 mg/mL corresponds to 3.5 mg/mL originally.
It is a dimensionless volume ratio. The accompanying concentration result applies mass balance and any recovery assumption entered.
Yes for a final-to-initial volume ratio. A value below 1 means the final volume is smaller and the material is concentrated.
Yes. Ratios are valid only when the volume inputs use the same unit.
Multiply the factor from each sequential step, keeping the direction of each ratio consistent.
A pure volume dilution factor does not. Where available, use the recovery input to account for processing loss separately.
| Field | Meaning |
|---|---|
| Original | Calculated output |
| Total \1Olume | Calculated output |
| Sample \1Raction | Calculated output |