Calculate the buffer or reagent volume needed for a centrifuge batch from tube count, fill volume, reagent fraction, and preparation overage. The breakdown distinguishes total tube fill from the reagent component so density media, wash buffer, or other liquid can be planned explicitly.
The main result is preparation volume for the selected reagent, not the total capacity rating of the tubes or rotor.
Confirm tube fill limits, balance opposing positions by mass, and follow the rotor and centrifuge manufacturer’s operating instructions.
For 24 tubes filled to 15 mL, with reagent making up 100% of the fill and 8% overage, the base is 360 mL and the preparation volume is 388.8 mL.
Overage covers expected pipetting, transfer, and vessel losses. It should reflect the workflow rather than being treated as a universal recommendation.
It is the total selected reagent or working mixture described by the inputs. Component ratios must still follow the relevant protocol.
Use the units shown in the fields. Convert inputs first so every volume in the same calculation uses the stated unit.
No. Confirm well, tube, rotor, and vessel limits separately before preparing or loading material.
Separating base demand from extra volume makes the preparation assumption visible and easier to document.
| Field | Meaning |
|---|---|
| Base | Calculated output |
| Extra | Calculated output |
| Batch \1Ill | Calculated output |