Serial Dilution Concentration Calculator

How to use this calculator

1. Enter the inputs using consistent units.
2. Review rate, timing, and scope assumptions.
3. Select Calculate to update every result.
4. Change one assumption at a time to compare scenarios.

Formula

The same transfer and diluent volumes are assumed at every step.

What the result means

The main result is the expected analyte concentration in the last vessel, before accounting for pipetting uncertainty, incomplete mixing, adsorption, or degradation.

This theoretical estimate assumes accurate transfers and complete mixing; laboratory results should be verified with appropriate controls and measurement uncertainty.

Example calculation

Starting at 100 units with a 1 mL transfer into 9 mL diluent gives a 1:10 step. Six steps produce a 1:1,000,000 total dilution and a final concentration of 0.0001 units.

Tips for better results

• Keep units consistent from input through result.
• Use unrounded values during the calculation and round only the displayed result.
• Run a conservative and an optimistic scenario before acting.
• Document assumptions so the estimate can be reproduced.