How to use this calculator
- Enter the inputs using consistent units.
- Review rate, timing, and scope assumptions.
- Select Calculate to update every result.
- Change one assumption at a time to compare scenarios.
#3303 · Science & Engineering Tool
Serial Dilution Sample Capacity Calculator
Plan how many samples a serial-dilution setup can support from available diluent and transfer material. The estimate identifies the limiting resource, total vessels, material used, and leftover capacity for a repeated dilution protocol.
Calculator
Enter assumptionsFormula
Capacity = floor[min(D ÷ (d × n), S ÷ s)]
D is available diluent, d is diluent per step, n is steps, S is source sample, and s is source sample per series.
What the result means
Capacity counts only complete sample series. It excludes overage for dead volume, repeats, controls, standards, and accidental loss.
This theoretical estimate assumes accurate transfers and complete mixing; laboratory results should be verified with appropriate controls and measurement uncertainty.
Example calculation
With 500 mL diluent, 9 mL per step, 6 steps, 25 mL sample, and 1 mL per series, diluent supports 9 series while sample supports 25. Capacity is therefore 9 series and 54 vessels.
Tips for better results
- Keep units consistent from input through result.
- Use unrounded values during the calculation and round only the displayed result.
- Run a conservative and an optimistic scenario before acting.
- Document assumptions so the estimate can be reproduced.
Frequently asked questions
Does capacity include controls and blank tubes?
No. Add those separately or reduce the available material entered.
Why is capacity rounded down?
A partial series cannot complete every planned dilution step.
Does each later transfer consume more source sample?
No. The model counts source sample only for the first aliquot of each series.
How do I allow for pipette dead volume?
Subtract the expected dead volume from available material before calculating.
Can the calculator plan different step volumes?
No. It assumes the same diluent volume at every step in a series.
Related calculators
Capacity planning variables
| Resource | Consumption per series |
|---|---|
| Diluent | Diluent per step × number of steps |
| Source sample | Initial transfer per series |
| Vessels | Capacity × steps |