Estimate how strongly centrifugation concentrates a sample after supernatant removal and resuspension. Enter the starting volume and concentration, the recovered fraction, and the final resuspension volume. The calculator reports the expected final concentration, concentration factor, recovered amount, and material lost so you can plan a practical pellet resuspension.
The main result is the expected concentration in the resuspended sample. It includes recovery loss, so it is more realistic than simply dividing the starting volume by the final volume.
This is a mass-balance estimate. Actual recovery depends on pellet formation, aspiration loss, adsorption, solubility, and resuspension completeness.
For 50 mL at 2 mg/mL, the starting amount is 100 mg. At 85% recovery, 85 mg remains. Resuspending in 5 mL gives 17 mg/mL, an 8.5× concentration factor, with 15 mg estimated loss.
The volume ratio ignores recovery loss. This calculator applies the recovered fraction before dividing by final volume.
No. Values above 100% usually indicate measurement variability, unit mismatch, or an incorrect blank.
Use 100% for a theoretical upper bound, then compare it with a conservative scenario such as 70% or 80%.
Yes. Residual supernatant increases the true final volume and can lower concentration relative to the estimate.
Yes, if both starting and final concentration are expressed in the same count-per-volume unit.
| Variable | Meaning | Unit |
|---|---|---|
| V₀ | Starting sample volume | mL |
| C₀ | Starting concentration | mg/mL |
| r | Recovered fraction | decimal |
| V₁ | Resuspension volume | mL |